What priority area of science and technology does it correspond to: technologies for creating molecular diagnostic systems and therapeutic agents, enzyme and bacterial preparations.
Research: applied.
Future application perspective: completed study.
Research level: no analogues in Ukraine.
Availability of a patent: 1 patent of Ukraine.
What additional actions require further research: additional funding.
Brief description, advantages, further perspective.
The effect of alkali and alkaline earth metal ions on the proteolytic activity of a new enzyme preparation, Bacillus thuringiensis var israelensis proteinase IMV B-7465, was studied. It was found that such metal ions as Li+, Ca2+, Sr2+, Mn2+ and Co2+ are peptidase activators and increase the caseinolytic activity of the enzyme by 5-22%, while Cu2+, Be2+, Cd2+, Hg2+, Cr3+, Fe3+ inhibit it.
Using the random forest method (RandomForest), an adequate QSAR model for predicting proteolytic activity was obtained using 4 descriptors: effective nuclear charge for the valence state of atoms; electronegativity of elements on the Pauling scale; electron affinity energy and entropy of ions in aqueous solution.
The polymer matrix for the immobilization of peptidase from Bacillusthuringiensisvar israelensis IMO B-7465 was improved by adding glycerin, which improved the elasticity of the coating and prevented the formation of cracks and other mechanical damage. Additionally, the content of chitosan was increased in the ratio with polyvinyl alcohol 1:5, while maintaining the peptidase activity at the level of 71.25% of the original. The effect of plasticizers used in pharmacy on the physical and mechanical characteristics of a dressing material with proteolytic action (tween-80, glycerin, triethylene glycol, triacetin) was studied.
Studies of the antibacterial properties of polymeric compositions PVA/chitosan/plasticizer and PVA/chitosan/plasticizer/enzyme on test strains of standard typical museum cultures of microorganisms: Staphylococcusaureus ATCC 25923 F-49; Escherichia coli 055 K59; Pseudomonasaeruginosa 415; Candida albicans ATCC 885-653 showed the need to add antiseptic agents to the composition.
The additional introduction of chlorhexidine bigluconate supplemented the spectrum of various types of enzyme activity with an effective bacteriostatic effect on the test strains under study.
The experimental data obtained can be used for further research into the creation of an innovative agent for the treatment of skin injuries (wounds and burns), including in military field conditions and disaster medicine.