R&D registration number in UkrISTEI: 0116U003464
Year of study start: 2020
Source of funding: implementation of initiative GDRs (developments) free of charge
Amount of financing: without financing
Level of innovation: no analogues in Ukraine
Availability of a patent: 3 patents of Ukraine.
Implementation: planned for 2024
Additional actions that require further implementation: expanding the range of research and updating the material and technical base; other. At this stage, research continues to improve and develop methods for determining toxicity by a microbiological express method using the Colpoda steinii infusoria for other food products and raw materials. After their completion, these methods will be included in the monitoring system and the entire developed complex will be implemented.
Brief description, technical and economic parameters of advantages and positive qualities.
The proposed method for determining toxicity relates to veterinary medicine, namely to microbiological methods for determining toxicity, and can be used when conducting an examination of the safety of cephalopod meat.
The method includes the preparation of a test culture of the ciliate Colpoda steinii., selection and preparation of a sample of the test product, its extraction with chemically pure acetone, filtering the extract, diluting the resulting filtrate, adding the resulting solution of acetone extract to the test culture of ciliates, thermostating the resulting mixture. Determination of the degree of toxicity is carried out by observing the vital activity of ciliates under a microscope at an increase of 80-120 and taking into account the number of living and dead ciliates. If the product is non-toxic, then at least 90% of Colpoda steinii ciliates are alive and actively moving. A decrease in activity and (or) death of ciliates indicates the toxicity of the product.
The method makes it possible to detect in the meat of cephalopods (octopus, squid and cuttlefish) a wide range of toxicants isolated by acetone (pesticides, salts of heavy metals, antibiotics, etc.), as well as to reduce the examination time to 1 hour, which will help facilitate, accelerate and acceleration. study of their toxicity.