R&D registration number in UkrISTEI: 0116U003464
Year of study start: 2020
Planned field of application and specific place of application: veterinary medicine; laboratories of the State Service of Ukraine for Food Safety and Consumer Protection.
Source of funding: implementation of initiative GDRs (developments) free of charge
Amount of financing: without financing
Level of innovation: no analogues in Ukraine
Availability of a patent: 1 patent of Ukraine.
Availability of an interested customer: laboratories of the State Service of Ukraine for Food Safety and Consumer Protection
Additional actions that require further implementation: expanding the range of research and updating the material and technical base; other. At this stage, research continues to improve and develop methods for determining toxicity by a microbiological express method using the Colpoda steinii infusoria for other food products and raw materials. After their completion, these methods will be included in the monitoring system and the entire developed complex will be introduced.
Brief description, technical and economic parameters of advantages and positive qualities.
The proposed method for determining toxicity relates to veterinary medicine, namely to microbiological methods for determining toxicity, and can be used when conducting an examination of the safety of meat from slaughtered animals. The method includes preparation of a test culture of the ciliate Colpoda steinii., selection and preparation of a sample of the test product, its extraction with chemically pure acetone, taken in a volume of 6-10 cm3, filtering the extract, diluting the resulting filtrate with Lozin-Lozinsky’s solution, adding the resulting solution of acetone extract to the test culture of ciliates, thermostating the resulting mixture at a temperature of +26 … +28 ° C for 60 minutes. Determination of the degree of toxicity is carried out by observing the vital activity of ciliates under a microscope at an increase of 80-120 and taking into account the number of living and dead ciliates. If the product is non-toxic, then at least 90% of Colpoda steinii ciliates are alive and actively moving. A decrease in activity and (or) death of ciliates indicates the toxicity of the product. Therefore, the method is efficient and easy to use and allows to detect a wide range of toxicants in the meat of slaughtered animals (lamb, pork, beef): pesticides, salts of heavy metals, antibiotics, alkaloid bases, glycosides and their aglycones, flavones and their aglycones, coumarins, carotenoids , vitamins of groups B, P, PP, essential oils, pigments, etc., within 1 hour. And thanks to the calculated and adapted ratios of the components, the method allows to conduct research on the toxicity of meat of varying degrees of cold treatment, which will facilitate and improve the study of its toxicity. Also, given the high sensitivity of the test culture used to low doses of toxicants, the method makes it possible to identify the possible total effect of subdoses of several toxicants at once and to establish the overall toxicity of the test sample.