The use of the invention will make it possible to develop a method for the early diagnosis of tuberculosis. Immunization of animals, selective cultivation of microorganisms, obtaining polyclonal antisera, affinity purification of preparations of polyclonal antisera, heterogeneous enzyme-linked immunosorbent assay. The model makes it possible to develop a method for diagnosing tuberculosis.
- 2-20 times less time in comparison with analogs;
- technical simplicity;
- selective accumulation of mycobacterial biomass without an intermediate stage of isolation of a pure culture of bacteria.
The method is developed on the basis of a vaccine strain, which is pretreated with a growth stimulant followed by its cultivation on a selective VKH medium.
The development is intended for the introduction of a safe and technically easy-to-implement method of obtaining immunogens from mycobacterium tuberculosis.
Medicine and immunology.
Provides consistent results.
Ready for implementation.
Sale of licenses. Collaborative industrialization.
1 patent of Ukraine.