Analysis of the consequences of interspecific introgressive hybridization using DNA microsatellites (on the example of the southern beef cattle)

What priority area of ​​science and technology does it correspond to: rational environmental management

Research: fundamental

Prospect for further implementation: to be continued as fundamental.

Research level: no analogues in Ukraine.

Availability of a patent: 1 patent of Ukraine.

What additional actions require further research: dissemination.

Brief description, advantages, further prospects for application. The main goal of the work was to study the genetic structure of the southern beef breed (LBM) of cattle and evaluate the taurine/zebuin impurities in the LBM population using DNA microsatellites. The study included 192 heifers: LZ (≤3/8 blood per zebu; 100 heads) and group UZ (>3/8 blood per zebu; 92 heads). To assess various parameters of genetic diversity, autosomal polymorphic microsatellite loci (BM1818, BM1824, BM2113, ETH3, ETH10, INRA023, TGLA53, TGLA122, TGLA227, and SPS115) were genotyped. The total number of genotypes significantly varied by loci from 18 (BM1824 locus) to 37 (INRA023 locus), with an average of 27.9±1.96 genotypes per locus. In general, one hundred and four alleles were noted for the studied microsatellite markers, however, the allelic diversity (average number of alleles per locus) was 10.4±0.76. For each locus, certain alleles were noted that were present at high frequencies in LZ animals and absent (or had relatively low frequencies) in UZ animals, or vice versa. An analysis of the nature of the relationship between certain alleles for each locus and indicus/taurus group-specificity was assessed based on a logistic regression model. A significant relationship was noted only for two loci – TGLA227 (χ2 = 22.30; P < 0.001) and ETH10 (χ2 = 27.70; P < 0.001). It can be assumed that the alleles TGLA227 (77 bp) and ETH10 (209-211 bp) among the studied individuals of cattle were inherited from the B.indicus ancestor. On the other hand, the alleles TGLA227 (89 bp) and ETH10 (217-219 bp), which are predominant among individuals of the LZ group, were inherited from the ancestor of B.taurus.


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