Prospects for further implementation: will be continued as applied.
Research level: no analogues in Ukraine.
Patent availability: 6 patents of Ukraine.
What additional actions require further research: the results of the study will be used in healthcare practice for the prevention and treatment of oral tissue diseases in patients with hepatobiliary pathology.
Brief description, advantages, further prospects for application.
During the implementation of the GDR, 16 series of experiments were performed using 276 Wistar rats, 592 patients were examined, 9400 biochemical and 1028 immunological analyzes were performed. The results of research have been published in scientific journals in the form of 68 articles, 1 textbook, 31 abstracts of conference and congress materials, 3 methodological recommendations, ¦6 – patents, 5 innovations, 3 projects of innovations are presented, set out in 8 speeches on television.
Simulation of various pathological conditions (hyposallation, inflammation, antibiotics, endotoxins, anti-Heliobacter complex, immunosuppression, lipid peroxides) in laboratory rats caused a decrease in the activity of lysozyme in the blood serum and mucous membranes of the oral cavity and intestines by 9-72. A decrease in lysozyme under the influence of experimental pathogenic factors leads to increased reproduction of opportunistic and pathogenic microbiota (an increase in urease activity by 27-64%) and the development of a dysbiotic syndrome, which subsequently initiates non-communicable diseases, including dental ones.
The universality of the development of dysbiosis in any non-infectious diseases realized through the influence of lipopolysaccharide on the activity of tissue lysozyme has been established. The conducted studies supplemented the information obtained about the leading role of the decrease in the antimicrobial function of the liver in the pathogenesis of generalized and local dysbiosis. The concept of a new nosological form of dysbiotic syndrome is substantiated.
The conducted studies revealed the main mechanism of the therapeutic action of lysozyme, namely, its ability to bind lipopolysaccharide into an inactive complex.
The pathogenetic role of polyfunctional antidysbiotic agents, including those first developed in the prevention and treatment of non-communicable diseases, including dental ones, is shown. The developed polyfunctional antidysbiotic drugs Lequin (tablets, powder) and Lysozym-forte (tablets, powder) were studied experimentally on 2 models (toxic hepatitis, peroxide periodontitis) and in the clinic on 547 patients with a gastroenterological pathologist. gastroduodenitis, hepatitis, colitis), their stomatotropic effect was determined.
In clinical studies, the effectiveness of dental prophylaxis using Lequin gel in patients with gastroduodenitis and hepato-biliary pathology, as well as the drug Lysozym-forte in patients with gastritis and gastroduodenitis, has been established.
Studies of the therapeutic and prophylactic effect of the gel “Dubovoy” were carried out on the model of experimental toxic hepatitis in rats and in the clinic.
In the course of the research, a clinical and statistical analysis of the indicators of the general immunity of patients with various forms of iatrogenic maxillary sinusitis was carried out. Clinical and statistical analysis of the parameters of general immunity in patients with iatrogenic maxillary sinusitis found that the traumatic form is characterized by an increase in the level of IgE, the infectious-allergic form is characterized by an increase in the values of cytotoxic T cells (CD3+, CD56+), and long-term sluggish drug and IgG with a decrease in circulating immune complexes in blood.
Practical value Reasonable expediency of using the method of determining the activity of urease to assess the microbial contamination of organs and tissues, the method of studying the activity of lysozyme to determine the degree of decrease in the level of the nonspecific immune system, the method of studying the activity of elastase to assess the severity of the inflammatory process.