Technology for obtaining a combination of metabolites of probiotic strains of fungi and bacteria

Purpose. To improve the method of obtaining a postbiotic agent with enhanced antibacterial action, which does not contain components of nutrient media, intended for use in various dosage forms in the treatment of infectious and inflammatory diseases.

Specifications. Disintegration parameters: ultrasonic generator GZ-109, loaded on a ring piezoceramic transducer-emitter of the TsTS type. Irradiation is carried out in an aqueous environment in a ring generator device in the frequency range Δf2=3550 kHz (fmax=40.0 kHz) with excitation amplitude U=15 V at a load R=50 Ω (P=5 W). The conversion coefficient of electrical to acoustic power is  5%, the average power of acoustic vibrations at the location of biological objects reaches (0.250.5) W. Concentrating the disintegrate: evaporation at 56 C for 30 minutes. Warming up the disintegrate: at 80 C for 40 minutes. Inoculum: 10% of the total volume, microbial suspension ratio 1:1, optical density – 10 units on the McFarland scale. Cultivation: at 37 °C for three days. Culture centrifugation parameters: 1000 g for 30 minutes. Membrane filter pore diameter: 0.2 microns.

Application area. Medicine, pharmacy, biotechnology. Biotechnological enterprises of various forms of ownership.

Advantages. Compared with known analogues, the proposed technology simplifies the procedure and reduces the time required to obtain a cell-free postbiotic product, enhances its antibacterial effect due to the expansion of the spectrum of probiotic derivatives with antimicrobial activity, reduces the risk of developing allergic reactions and prevents the transmission of prion infections due to the product.

Technical and economic effect. The technical and economic effect of using the technology is to simplify and reduce the cost of the procedure for obtaining the target product by halving the number of technological steps and eliminating the need to use traditional nutrient media. The social effect is to enhance up to 2.4 times the antibacterial (anti-adhesive, anti-biofilm and inhibitory) effect of the final product against pathogenic and opportunistic bacteria due to the co-cultivation of probiotic microorganisms, which is usually accompanied by an expansion of the spectrum of antimicrobial substances produced by them. The use of a postbiotic product with an enhanced antibacterial effect, free from the components of the nutrient medium, will help to increase the effectiveness of the treatment of infectious and inflammatory diseases.

Description. The essence of the technology is to obtain, without the use of traditional nutrient media, from probiotic strains of bacteria Lactobacillus rhamnosus GG and fungi Saccharomyces boulardii, a cell-free postbiotic product with enhanced antibacterial effect by expanding the spectrum of produced lactobacilli. lactobacilli. A technology is proposed for producing a cell-free postbiotic product free from nutrient media components based on a combination of structural components and metabolites of probiotic bacteria Lactobacillus rhamnosus GG and fungi Saccharomyces boulardii with enhanced antibacterial action. bacteria followed by removal of cells and cellular debris. The use of such a postbiotic in various dosage forms makes it possible to increase the effectiveness of treatment of infectious and inflammatory diseases.

More details…