Method for obtaining a complex of papain with arabinoxylan

R&D registration number in UkrISTEI: 0117U000362

What priority area of ​​science and technology does it correspond to: fundamental scientific research on the most important problems of developing scientific, technical, socio-economic, socio-political, human potential to ensure the competitiveness of Ukraine in the world and the sustainable development of society and the state

Research: targeted fundamental

Source of funding: funds from the general fund of the state budget.

Research level: no analogues in Ukraine.

Availability of a patent: 1 patent of Ukraine.

What additional actions require further research: further research requires the approval of regulatory documentation and the conduct of pharmacological and toxicological studies of products, this requires additional funding.

Brief description, advantages, further prospects for application.

The diet of the population of Ukraine is deficient in the content of a number of biologically active substances, but their direct addition to food is ineffective. The expediency of the formation of a complex of papain with corn embryo arabinoxylan as a means of directed modification of the enzyme properties has been proved. The rational conditions for obtaining the complex, which significantly exceeds the initial papain in activity, are determined:

mixing solutions of a polysaccharide with a concentration of 0.25% and an enzyme with a concentration of 0.4% in a volume ratio of 1:1 at pH 6 at room temperature for 20 minutes. The preparation of the complex was confirmed by thermogravimetry. The inclusion of papain in the composition of the complex makes it possible to increase its resistance to changes in pH and temperature. In the range of physiological pH values, the activity of the enzyme in the complex is higher than that of free papain. Complexation also increases the stability of the enzyme at elevated temperatures. The papain-arabinoxylan complex is a dietary supplement that is advisable to use to correct digestion processes.

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